Electron Microscopy of Poliomyelitis Virus
نویسندگان
چکیده
The infectious capacity of poliomyelitis virus has been shown to be associated with spherical particles approximately 30 mim in diameter.7"0"l'17 The present study suggests that these particles, like those of other animal viruses recently studied8'8'9 consist of an outer membranous structure surrounding an internal nucleus-like body. The ultimate size and shape of small virus particles as they are seen in shadowed electron micrographs appear to be dependent upon the hydration of the particles at the time of the first exposure to high vacuum. By the use of a collodion-over-agar filtration technique it has been possible to prepare and mount virus suspensions directly from saline media and obtain microscope specimens which are free of salt crystals and osmotic distortion. Such preparations preserve the three-dimensional structure of the cell-like particles. Through variations in the drying time, the saline media, and through inclusion of hygroscopic substances in the media, the degree of hydration prior to the initial vacuum drying can be altered. Representative strains of poliomyelitis virus (Mahoney, Type 1; MEF-1, Type 2; and Saukett, Type 3) have been studied; comparisons of shadowed and unshadowed electron micrographs have revealed internal detail and morphology within poliomyelitis virus particles.
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عنوان ژورنال:
- The Yale Journal of Biology and Medicine
دوره 28 شماره
صفحات -
تاریخ انتشار 1956